I think we've ordered 138 at the moment. But the original order was only for 48 - i.e. one air group. However you would expect any warship to be out of commission for maintenance, repairs and refit for about 20-30% of its lifetime
Given the complexity of the F35, the availability of the aircraft itself is going to be even poorer than anything preceding it. And, as the crash record of the Harrier showed, S/VTOL aircraft tend to have dreadful accident rates, whether through pilot error, technical failure, or other hazards like FOD and bird strikes that seem to be more significant for S/VTOL types.