Yes you can, but probaby not on a Sonos speaker
When I had an OK stereo I had to re-rip all of my 196kbps to 320kbps as the OK stereo made the differences stand out like a sore thumb.
Then when I upgraded to a Cyrus set and B&W speakers I had to re-rip it all to FLAC becuase after searching for a problem in my new set-up for a couple of hours as the sound from my iPod classic sucked I eventually did a comparison with the CD and it was night and day different.
The old stereo was relegated to the bedroom to use as an alarm clock, and MP3s still sound fine on that system, you can't tell them from the CDs they came on.
I now have a Sonos connect connected to the preamp with a coax digital cable and it plays the FLACs from a network share. It sounds way better than I expected it to, to the point where the CDs were taken out of the living room the same day.
But after listening to Sonos speakers at friends and in shops I wouldn't expect you to see the difference from the 196 to FLAC, let alone the 320...
(Oh, and to answer the question about cables from someone earlier: I use the mains cables that came with the units, and cheap, but very thick, speaker cable as reccomended by the shop I got the speakers from - they commented that the expensive cable is for showing off, not for the music)